GPB-212 Principles of Genetics New Syllabus 6th Dean’s

GPB-212 Principles of Genetics New Syllabus 6th Dean’s

Objectie

To make the students acquainted with both principles and practices in the areas of classical genetics, modern genetics, quantitative genetics and cytogenetics.

Theory

Pre and post Mendelian concepts of heredity, Mendelian principles of heredity, Study of model organisms (Drosophila, Arabidopsis, Garden pea, E. coli, and mice), Architecture of chromosomes, chromonemata, chromosome matrix, chromomeres, centromere, secondary constriction and telomere, special types of chromosomes, Chromosomal theory of inheritance- cell cycle and cell division-mitosis and meiosis. Probabilit and Chi-square. Types of DNA and RNA, Dominance relationships, Epistatic interactions with example, Introduction and definition of cytology, genetics and cytogenetics and their interrelation.
Multiple alleles, pleiotropism and pseudoalleles, Sex determination and sex linkage, sex limited and sex influenced traits, Blood group genetics, Linkage and its estimation, crossing over mechanism, chromosome mapping, Structural and numerical variations in chromosomes and their implications, Use of haploids, dihaploids and double haploids in Genetics, Mutation, classification, Methods of inducing mutations, mutagenic agents and induction of mutation. Qualitative and quantitative traits, Polygenes and continuous variations, multiple factor hypothesis, Cytoplasmic inheritance, Nature, structure and replication of genetic material, Protein synthesis, Transcription and translational mechanism of genetic material, Gene concept: Gene structure, function and regulation.

Practical

Study of microscope, Study of cell structure, Mitosis and Meiosis cell division, Experiments on monohybrid, dihybrid, trihybrid, test cross and back cross, Experiments on epistatic interactions including test cross and back cross, Practice on mitotic and meiotic cell division, Experiments on probability and chi-square test, Determination of linkage and croo-over analysis (through two point test cross data), Study on sex linked inheritance in Drosophila. Study on models on DNA and RNA structures.

Lecture Schedule: Theory

1. Pre and Post Mendelian concepts of heredity 1
2. Mendelian principles of heredity 1
3. Cell division – mitosis 1
4. Cell division – meiosis 1
5. Probability and Chi-square 1
6. Dominance relationships and gene interaction 1
7. Epistatic gene interactions with examples (complementary, supplementary, duplicate gene interactions) 1
8. Epistatic gene interactions with examples (masking, inhibitory, polymeric and additive gene interactions) 1
9. Pleiotropism, pseudoalleles, Multiple alleles and Blood group genetics 1
10. Sex determination 1
11. Sex limited, sex influenced and sex linked traits 1
12. Sex linkage 1
13. Linkage and its estimation 1
14. Crossing over : introduction & mechanisms 1
15. Chromosome mapping 1
16. Structural changes in chromosome 1
17. Numerical changes in chromosome 1
18. Mutation: introduction, characteristics & classification 1
19. Mutagenic agents: physical and chemical mutagens 1
20. Induction of mutation, Methods of inducing mutation & CIB technique 1
21. Qualitative & Quantitative traits, Polygenes and continuous variations 1
22. Multiple factor hypothesis 1
23. Cytoplasmic inheritance 1
24. Genetic disorders 1
25. Nature, structure and types of genetic material 1
26. Proof for DNA as genetic material 1
27. Replication of genetic material 1
28. Genetic code & Protein synthesis 1
29. Transcription mechanism of genetic material 1
30. Translational mechanism of genetic material 1
31. Gene concept: Gene structure and function 1
32. Gene regulation, operon concept, Lac and Trp operons 1

Lecture Schedule: Practical

1. Study of microscope: parts and types 1
2. Study of cell structure 1
3. Experiments on monohybrid, test cross and back cross 1
4. Experiments on dihybrid, test cross and back cross 1
5. Experiments on trihybrid, test cross and back cross 1
6. Experiments on epistatic interactions including test cross and back cross 1
7. Experiments on epistatic interactions including test cross and back cross 1
8. Stains and their preparation 1
9. Fixatives and their preparation 1
10. Practice on mitotic cell division 1
11. Practice on meiotic cell division 1
12. Experiments on probability 1
13. Experiments on Chi-square test 1
14. Determination of linkage and cross over analysis (through two-point test cross and three-point test cross data) 1
15. Study on sex linked inheritance in Drosophila 1
16. Study of models on DNA and RNA structure 1

Suggested readings

1. Fundamentals of Genetics: B. D. Singh
2. Genetics: M. W. Strickberger.
3. Principles of Genetics: Gardner, Simmons and Snustad.
4. Principles of Genetics: Sinnott, Dunn and Dobzhansky